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Structure‐guided design of a reversible fluorogenic reporter of protein‐protein interactions
A reversible green fluorogenic protein‐fragment complementation assay was developed based on the crystal structure of UnaG, a recently discovered fluorescent protein. In living mammalian cells, the nonfluorescent fragments complemented and rapidly became fluorescent upon rapamycin‐induced FKBP and F...
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| Publicado no: | Protein Sci |
|---|---|
| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
John Wiley and Sons Inc.
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4815423/ https://ncbi.nlm.nih.gov/pubmed/26690964 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2866 |
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