Use of a yeast expression system for the isolation and analysis of drug-resistant mutants of a mammalian phosphodiesterase.
Saccharomyces cerevisiae strain PP5 has a phosphodiesterase (PDE) deficiency that results in heat-shock sensitivity due to the intracellular accumulation of cAMP. This strain also carries the cam mutation, which confers permeability to cAMP and, as shown here, to other compounds. Expression of rat t...
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| Pubblicato in: | Proc Natl Acad Sci U S A |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
1993
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC48107/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7505450/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.24.11970 |
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