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The Ec-NhaA antiporter switches from antagonistic to synergistic antiport upon a single point mutation

The Na(+), Li(+)/H(+) antiporter of Escherichia coli (Ec-NhaA) maintains pH, Na(+) homeostasis in enterobacteria. We used isothermal titration calorimetry to perform a detailed thermodynamic analysis of Li(+) binding to Ec-NhaA and several of its mutants. We found that, in line with the canonical al...

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Foilsithe in:Sci Rep
Main Authors: Dwivedi, Manish, Sukenik, Shahar, Friedler, Assaf, Padan, Etana
Formáid: Artigo
Teanga:Inglês
Foilsithe: Nature Publishing Group 2016
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Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4810432/
https://ncbi.nlm.nih.gov/pubmed/27021484
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep23339
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