Application and Optimization of relE as a Negative Selection Marker for Making Definitive Genetic Constructs in Uropathogenic Escherichia coli

Studies of Uropathogenic Escherichia coli (UPEC) pathogenesis have relied heavily on genetic manipulation to understand virulence factors. We applied a recently reported positive-negative selection system to create a series of unmarked, scarless FimH mutants that show identical phenotypes to previou...

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Detaylı Bibliyografya
Yayımlandı:Pathogens
Asıl Yazarlar: Khetrapal, Varnica, Mehershahi, Kurosh S., Chen, Siyi, Chen, Swaine L.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: MDPI 2016
Konular:
Communication
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4810130/
https://ncbi.nlm.nih.gov/pubmed/26797639
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/pathogens5010009
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