Application and Optimization of relE as a Negative Selection Marker for Making Definitive Genetic Constructs in Uropathogenic Escherichia coli

Studies of Uropathogenic Escherichia coli (UPEC) pathogenesis have relied heavily on genetic manipulation to understand virulence factors. We applied a recently reported positive-negative selection system to create a series of unmarked, scarless FimH mutants that show identical phenotypes to previou...

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Hlavní autoři: Varnica Khetrapal, Kurosh S. Mehershahi, Siyi Chen, Swaine L. Chen
Médium: Artigo
Jazyk:Inglês
Vydáno: MDPI AG 2016-01-01
Edice:Pathogens
Témata:
negative selection
UTI89
FimH
On-line přístup:http://www.mdpi.com/2076-0817/5/1/9
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