Application and Optimization of relE as a Negative Selection Marker for Making Definitive Genetic Constructs in Uropathogenic Escherichia coli

Studies of Uropathogenic Escherichia coli (UPEC) pathogenesis have relied heavily on genetic manipulation to understand virulence factors. We applied a recently reported positive-negative selection system to create a series of unmarked, scarless FimH mutants that show identical phenotypes to previou...

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Bibliografiska uppgifter
Huvudupphovsmän: Varnica Khetrapal, Kurosh S. Mehershahi, Siyi Chen, Swaine L. Chen
Materialtyp: Artigo
Språk:Inglês
Publicerad: MDPI AG 2016-01-01
Serie:Pathogens
Ämnen:
negative selection
UTI89
FimH
Länkar:http://www.mdpi.com/2076-0817/5/1/9
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