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RPAP1, a Novel Human RNA Polymerase II-Associated Protein Affinity Purified with Recombinant Wild-Type and Mutated Polymerase Subunits

We have programmed human cells to express physiological levels of recombinant RNA polymerase II (RNAPII) subunits carrying tandem affinity purification (TAP) tags. Double-affinity chromatography allowed for the simple and efficient isolation of a complex containing all 12 RNAPII subunits, the genera...

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Guardat en:
Publicat a:Mol Cell Biol
Autors principals: Jeronimo, Célia, Langelier, Marie-France, Zeghouf, Mahel, Cojocaru, Marilena, Bergeron, Dominique, Baali, Dania, Forget, Diane, Mnaimneh, Sanie, Davierwala, Armaity P., Pootoolal, Jeff, Chandy, Mark, Canadien, Veronica, Beattie, Bryan K., Richards, Dawn P., Workman, Jerry L., Hughes, Timothy R., Greenblatt, Jack, Coulombe, Benoit
Format: Artigo
Idioma:Inglês
Publicat: Taylor & Francis 2004
Matèries:
Transcriptional Regulation
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC479746/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15282305/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/MCB.24.16.7043-7058.2004
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