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RPAP1, a Novel Human RNA Polymerase II-Associated Protein Affinity Purified with Recombinant Wild-Type and Mutated Polymerase Subunits
We have programmed human cells to express physiological levels of recombinant RNA polymerase II (RNAPII) subunits carrying tandem affinity purification (TAP) tags. Double-affinity chromatography allowed for the simple and efficient isolation of a complex containing all 12 RNAPII subunits, the genera...
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Main Authors: | , , , , , , , , , , , , , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado: |
American Society for Microbiology
2004
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Assuntos: | |
Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC479746/ https://ncbi.nlm.nih.gov/pubmed/15282305 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.24.16.7043-7058.2004 |
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