RPAP1, a Novel Human RNA Polymerase II-Associated Protein Affinity Purified with Recombinant Wild-Type and Mutated Polymerase Subunits

We have programmed human cells to express physiological levels of recombinant RNA polymerase II (RNAPII) subunits carrying tandem affinity purification (TAP) tags. Double-affinity chromatography allowed for the simple and efficient isolation of a complex containing all 12 RNAPII subunits, the genera...

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Detalhes bibliográficos
Main Authors: Jeronimo, Célia, Langelier, Marie-France, Zeghouf, Mahel, Cojocaru, Marilena, Bergeron, Dominique, Baali, Dania, Forget, Diane, Mnaimneh, Sanie, Davierwala, Armaity P., Pootoolal, Jeff, Chandy, Mark, Canadien, Veronica, Beattie, Bryan K., Richards, Dawn P., Workman, Jerry L., Hughes, Timothy R., Greenblatt, Jack, Coulombe, Benoit
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Transcriptional Regulation
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC479746/
https://ncbi.nlm.nih.gov/pubmed/15282305
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.24.16.7043-7058.2004
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