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Kinetic mechanism and fidelity of nick sealing by Escherichia coli NAD(+)-dependent DNA ligase (LigA)

Escherichia coli DNA ligase (EcoLigA) repairs 3′-OH/5′-PO(4) nicks in duplex DNA via reaction of LigA with NAD(+) to form a covalent LigA-(lysyl-Nζ)–AMP intermediate (step 1); transfer of AMP to the nick 5′-PO(4) to form an AppDNA intermediate (step 2); and attack of the nick 3′-OH on AppDNA to form...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Chauleau, Mathieu, Shuman, Stewart
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4797296/
https://ncbi.nlm.nih.gov/pubmed/26857547
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw049
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