Loading...
Kinetic mechanism and fidelity of nick sealing by Escherichia coli NAD(+)-dependent DNA ligase (LigA)
Escherichia coli DNA ligase (EcoLigA) repairs 3′-OH/5′-PO(4) nicks in duplex DNA via reaction of LigA with NAD(+) to form a covalent LigA-(lysyl-Nζ)–AMP intermediate (step 1); transfer of AMP to the nick 5′-PO(4) to form an AppDNA intermediate (step 2); and attack of the nick 3′-OH on AppDNA to form...
Saved in:
| Published in: | Nucleic Acids Res |
|---|---|
| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
2016
|
| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4797296/ https://ncbi.nlm.nih.gov/pubmed/26857547 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw049 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|