Organoid culture systems for prostate epithelial tissue and prostate cancer tissue

This protocol describes a recently developed strategy to generate 3D prostate organoid cultures from healthy mouse and human prostate (either bulk or FAC-sorted single luminal and basal cells), metastatic prostate cancer lesions and circulating tumour cells. Organoids derived from healthy material c...

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Veröffentlicht in:Nat Protoc
Hauptverfasser: Drost, Jarno, Karthaus, Wouter R., Gao, Dong, Driehuis, Else, Sawyers, Charles L., Chen, Yu, Clevers, Hans
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2016
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4793718/
https://ncbi.nlm.nih.gov/pubmed/26797458
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.006
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