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Yeast Helicase Pif1 Unwinds RNA:DNA Hybrids with Higher Processivity than DNA:DNA Duplexes

Saccharomyces cerevisiae Pif1, an SF1B helicase, has been implicated in both mitochondrial and nuclear functions. Here we have characterized the preference of Pif1 for RNA:DNA heteroduplexes in vitro by investigating several kinetic parameters associated with unwinding. We show that the preferential...

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Vydáno v:J Biol Chem
Hlavní autoři: Chib, Shubeena, Byrd, Alicia K., Raney, Kevin D.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Biochemistry and Molecular Biology 2016
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4786723/
https://ncbi.nlm.nih.gov/pubmed/26733194
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M115.688648
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