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Noncompetitive affinity assays of glucagon and amylin using mirror-image aptamers as affinity probes

The ability to detect picomolar concentrations of glucagon and amylin using fluorescently labeled mirror-image aptamers, so-called Spiegelmers, is demonstrated. Spiegelmers rival the specificity of antibodies and overcome the problem of biostability of natural aptamers in a biological matrix. Using...

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Detalles Bibliográficos
Publicado en:Analyst
Main Authors: Yi, Lian, Wang, Xue, Bethge, Lucas, Klussmann, Sven, Roper, Michael G.
Formato: Artigo
Idioma:Inglês
Publicado: 2016
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4783278/
https://ncbi.nlm.nih.gov/pubmed/26881276
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c5an02468d
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