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Spin labeling and Double Electron-Electron Resonance (DEER) to Deconstruct Conformational Ensembles of HIV Protease

An understanding of macromolecular conformational equilibrium in biological systems is oftentimes essential to understand function, dysfunction, and disease. For the past few years, our lab has been utilizing site-directed spin labeling (SDSL), coupled with electron paramagnetic resonance (EPR) spec...

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Detalhes bibliográficos
Publicado no:Methods Enzymol
Main Authors: Casey, Thomas M., Fanucci, Gail E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4783135/
https://ncbi.nlm.nih.gov/pubmed/26477251
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2015.07.019
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