Effect of Freezing Conditions on Distances and Their Distributions Derived from Double Electron Electron Resonance (DEER): A Study of Doubly-Spin-Labeled T4 Lysozyme

Pulsed dipolar ESR spectroscopy, DEER and DQC, require frozen samples. An important issue in the biological application of this technique is how the freezing rate and concentration of cryoprotectant could possibly affect the conformation of biomacromolecule and/or spin-label. We studied in detail th...

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Hauptverfasser: Georgieva, Elka R., Roy, Aritro S., Grigoryants, Vladimir M., Borbat, Petr P., Earle, Keith A., Scholes, Charles P., Freed, Jack H.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2012
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3323113/
https://ncbi.nlm.nih.gov/pubmed/22341208
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmr.2012.01.004
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