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Probing the promiscuity of ent-kaurene oxidases via combinatorial biosynthesis

The substrate specificity of enzymes from natural products’ metabolism is a topic of considerable interest, with potential biotechnological use implicit in the discovery of promiscuous enzymes. However, such studies are often limited by the availability of substrates and authentic standards for iden...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Mafu, Sibongile, Jia, Meirong, Zi, Jiachen, Morrone, Dana, Wu, Yisheng, Xu, Meimei, Hillwig, Matthew L., Peters, Reuben J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2016
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4780596/
https://ncbi.nlm.nih.gov/pubmed/26884192
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1512096113
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