Factors that Influence the Formation and Stability of Thin, Cryo-EM Specimens

Poor consistency of the ice thickness from one area of a cryo-electron microscope (cryo-EM) specimen grid to another, from one grid to the next, and from one type of specimen to another, motivates a reconsideration of how to best prepare suitably thin specimens. Here we first review the three relate...

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Udgivet i:Biophys J
Main Authors: Glaeser, Robert M., Han, Bong-Gyoon, Csencsits, Roseann, Killilea, Alison, Pulk, Arto, Cate, Jamie H.D.
Format: Artigo
Sprog:Inglês
Udgivet: The Biophysical Society 2016
Fag:
Biophysical Perspective
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4775786/
https://ncbi.nlm.nih.gov/pubmed/26386606
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2015.07.050
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