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Identification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID)
Proximity-dependent trans-biotinylation by the Escherichia coli biotin ligase BirA mutant R118G (BirA*) allows stringent streptavidin affinity purification of proximal proteins. This so-called BioID method provides an alternative to the widely used co-immunoprecipitation (co-IP) to identify protein-...
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| Udgivet i: | PLoS One |
|---|---|
| Main Authors: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Public Library of Science
2016
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4774922/ https://ncbi.nlm.nih.gov/pubmed/26934103 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0150239 |
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