Identification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID)

Proximity-dependent trans-biotinylation by the Escherichia coli biotin ligase BirA mutant R118G (BirA*) allows stringent streptavidin affinity purification of proximal proteins. This so-called BioID method provides an alternative to the widely used co-immunoprecipitation (co-IP) to identify protein-...

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Bibliographic Details
Published in:PLoS One
Main Authors: Schweingruber, Christoph, Soffientini, Paolo, Ruepp, Marc-David, Bachi, Angela, Mühlemann, Oliver
Format: Artigo
Language:Inglês
Published: Public Library of Science 2016
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4774922/
https://ncbi.nlm.nih.gov/pubmed/26934103
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0150239
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