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Methods for Characterization of Alternative RNA Splicing

Quantification of alternative splicing to detect the abundance of differentially spliced isoforms of a gene in total RNA can be accomplished via RT-PCR using both quantitative real-time and semi-quantitative PCR methods. These methods require careful PCR primer design to ensure specific detection of...

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書目詳細資料
發表在:Methods Mol Biol
Main Authors: Harvey, Samuel E., Cheng, Chonghui
格式: Artigo
語言:Inglês
出版: 2016
主題:
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4770877/
https://ncbi.nlm.nih.gov/pubmed/26721495
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-3378-5_18
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