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Methods for Characterization of Alternative RNA Splicing
Quantification of alternative splicing to detect the abundance of differentially spliced isoforms of a gene in total RNA can be accomplished via RT-PCR using both quantitative real-time and semi-quantitative PCR methods. These methods require careful PCR primer design to ensure specific detection of...
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| Published in: | Methods Mol Biol |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2016
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4770877/ https://ncbi.nlm.nih.gov/pubmed/26721495 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-3378-5_18 |
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