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Transient overexpression of DNA adenine methylase enables efficient and mobile genome engineering with reduced off-target effects
Homologous recombination of single-stranded oligonucleotides is a highly efficient process for introducing precise mutations into the genome of E. coli and other organisms when mismatch repair (MMR) is disabled. This can result in the rapid accumulation of off-target mutations that can mask desired...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4770203/ https://ncbi.nlm.nih.gov/pubmed/26496947 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv1090 |
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