Transient overexpression of DNA adenine methylase enables efficient and mobile genome engineering with reduced off-target effects

Homologous recombination of single-stranded oligonucleotides is a highly efficient process for introducing precise mutations into the genome of E. coli and other organisms when mismatch repair (MMR) is disabled. This can result in the rapid accumulation of off-target mutations that can mask desired...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Lennen, Rebecca M., Nilsson Wallin, Annika I., Pedersen, Margit, Bonde, Mads, Luo, Hao, Herrgård, Markus J., Sommer, Morten O. A.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2016
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Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4770203/
https://ncbi.nlm.nih.gov/pubmed/26496947
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv1090
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