Methanol-based fixation is superior to buffered formalin for next-generation sequencing of DNA from clinical cancer samples

BACKGROUND: Next-generation sequencing (NGS) of tumour samples is a critical component of personalised cancer treatment, but it requires high-quality DNA samples. Routine neutral-buffered formalin (NBF) fixation has detrimental effects on nucleic acids, causing low yields, as well as fragmentation a...

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Dettagli Bibliografici
Pubblicato in:Ann Oncol
Autori principali: Piskorz, A. M., Ennis, D., Macintyre, G., Goranova, T. E., Eldridge, M., Segui-Gracia, N., Valganon, M., Hoyle, A., Orange, C., Moore, L., Jimenez-Linan, M., Millan, D., McNeish, I. A., Brenton, J. D.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2016
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Original Articles
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4769995/
https://ncbi.nlm.nih.gov/pubmed/26681675
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/annonc/mdv613
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