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Methanol-based fixation is superior to buffered formalin for next-generation sequencing of DNA from clinical cancer samples
BACKGROUND: Next-generation sequencing (NGS) of tumour samples is a critical component of personalised cancer treatment, but it requires high-quality DNA samples. Routine neutral-buffered formalin (NBF) fixation has detrimental effects on nucleic acids, causing low yields, as well as fragmentation a...
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| Publicado no: | Ann Oncol |
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| Main Authors: | , , , , , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4769995/ https://ncbi.nlm.nih.gov/pubmed/26681675 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/annonc/mdv613 |
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