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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

The growing realization that both the temporal and spatial regulation of gene expression can have important consequences on cell function has led to the development of diverse techniques to visualize individual RNA transcripts in single living cells. One promising technique that has recently been de...

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Détails bibliographiques
Publié dans:J Vis Exp
Auteurs principaux: Song, Yang, Zhang, Xuemei, Huang, Lingyan, Behlke, Mark A., Tsourkas, Andrew
Format: Artigo
Langue:Inglês
Publié: MyJove Corporation 2014
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4758713/
https://ncbi.nlm.nih.gov/pubmed/25146531
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51544
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