Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

The growing realization that both the temporal and spatial regulation of gene expression can have important consequences on cell function has led to the development of diverse techniques to visualize individual RNA transcripts in single living cells. One promising technique that has recently been de...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Song, Yang, Zhang, Xuemei, Huang, Lingyan, Behlke, Mark A., Tsourkas, Andrew
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2014
Fag:
Genetics
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4758713/
https://ncbi.nlm.nih.gov/pubmed/25146531
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51544
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