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Site-specific protein modification using lipoic acid ligase and bis-aryl hydrazone formation

A screen of Trp37 mutants of E. coli lipoic acid ligase (LplA) produced enzymes capable of ligating an aryl-aldehyde or an aryl-hydrazine substrate to LplA’s 13-amino acid acceptor peptide (LAP). Once site-specifically attached to recombinant proteins, aryl-aldehydes could be chemo-selectively deriv...

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Foilsithe in:Chembiochem
Main Authors: Cohen, Justin D., Zou, Peng, Ting, Alice Y.
Formáid: Artigo
Teanga:Inglês
Foilsithe: 2012
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4758125/
https://ncbi.nlm.nih.gov/pubmed/22492621
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201100764
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