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A Modular Assembly Platform for Rapid Generation of DNA Constructs

Traditional cloning methods have limitations on the number of DNA fragments that can be simultaneously manipulated, which dramatically slows the pace of molecular assembly. Here we describe GMAP, a Gibson assembly-based modular assembly platform consisting of a collection of promoters and genes, whi...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Akama-Garren, Elliot H., Joshi, Nikhil S., Tammela, Tuomas, Chang, Gregory P., Wagner, Bethany L., Lee, Da-Yae, Rideout III, William M., Papagiannakopoulos, Thales, Xue, Wen, Jacks, Tyler
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4757859/
https://ncbi.nlm.nih.gov/pubmed/26887506
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep16836
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