A Modular Assembly Platform for Rapid Generation of DNA Constructs

Traditional cloning methods have limitations on the number of DNA fragments that can be simultaneously manipulated, which dramatically slows the pace of molecular assembly. Here we describe GMAP, a Gibson assembly-based modular assembly platform consisting of a collection of promoters and genes, whi...

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Pubblicato in:Sci Rep
Autori principali: Akama-Garren, Elliot H., Joshi, Nikhil S., Tammela, Tuomas, Chang, Gregory P., Wagner, Bethany L., Lee, Da-Yae, Rideout III, William M., Papagiannakopoulos, Thales, Xue, Wen, Jacks, Tyler
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group 2016
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4757859/
https://ncbi.nlm.nih.gov/pubmed/26887506
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep16836
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