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Modular Synthetic Inverters from Zinc Finger Proteins and Small RNAs

Synthetic zinc finger proteins (ZFPs) can be created to target promoter DNA sequences, repressing transcription. The binding of small RNA (sRNA) to ZFP mRNA creates an ultrasensitive response to generate higher effective Hill coefficients. Here we combined three “off the shelf” ZFPs and three sRNAs...

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Publié dans:PLoS One
Auteurs principaux: Hsia, Justin, Holtz, William J., Maharbiz, Michel M., Arcak, Murat, Keasling, Jay D.
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4757538/
https://ncbi.nlm.nih.gov/pubmed/26886888
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0149483
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