Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation

Nanobodies are single-domain antibodies of camelid origin. We generated nanobodies against the vertebrate nuclear pore complex (NPC) and used them in STORM imaging to locate individual NPC proteins with <2 nm epitope-label displacement. For this, we introduced cysteines at specific positions in t...

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Detalles Bibliográficos
Publicado en:eLife
Autores principales: Pleiner, Tino, Bates, Mark, Trakhanov, Sergei, Lee, Chung-Tien, Schliep, Jan Erik, Chug, Hema, Böhning, Marc, Stark, Holger, Urlaub, Henning, Görlich, Dirk
Formato: Artigo
Lenguaje:Inglês
Publicado: eLife Sciences Publications, Ltd 2015
Materias:
Biochemistry
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4755751/
https://ncbi.nlm.nih.gov/pubmed/26633879
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.11349
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