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Mechanistic studies of the radical SAM enzyme 4-demethylwyosine synthase reveals the site of hydrogen atom abstraction
TYW1 catalyzes the formation of 4-demethylwyosine via the condensation of N-methylguanosine (m(1)G) with carbons 2 and 3 of pyruvate. In this study labeled transfer ribonucleic acid (tRNA) and pyruvate were utilized to determine the site of hydrogen atom abstraction and regiochemistry of the pyruvat...
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| Publicado no: | Biochemistry |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4753065/ https://ncbi.nlm.nih.gov/pubmed/26052987 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.5b00476 |
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