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Ultracentrifugation-free chromatography-mediated large-scale purification of recombinant adeno-associated virus serotype 1 (rAAV1)

Recombinant adeno-associated virus (rAAV) is an attractive tool for gene transfer and shows potential for use in human gene therapies. The current methods for the production and purification of rAAV from the transfected cell lysate are mainly based on cesium chloride and iodixanol density ultracentr...

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Détails bibliographiques
Publié dans:Mol Ther Methods Clin Dev
Auteurs principaux: Tomono, Taro, Hirai, Yukihiko, Okada, Hironori, Adachi, Kumi, Ishii, Akiko, Shimada, Takashi, Onodera, Masafumi, Tamaoka, Akira, Okada, Takashi
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4748944/
https://ncbi.nlm.nih.gov/pubmed/26913289
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/mtm.2015.58
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