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Amplification-free In Situ KRAS Point Mutation Detection at 60 copies/mL in Urine in a Background of 1000-fold Wild Type

We have examined in situ detection of single-nucleotide KRAS mutation in urine using a (Pb(Mg(1/3)Nb(2/3))O(3))(0.65)(PbTiO(3))(0.35) (PMN-PT) piezoelectric plate sensor (PEPS) coated with a 17-nucleotide (nt) locked nucleic acid (LNA) probe DNA complementary to the KRAS mutation. To enhance in situ...

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Bibliografiska uppgifter
I publikationen:Analyst
Huvudupphovsmän: KirimLi, Ceyhun E., Shih, Wei-Heng, Shih, Wan Y.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2016
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4747796/
https://ncbi.nlm.nih.gov/pubmed/26783561
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c5an02048d
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