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Crystal structures and inhibition kinetics reveal a two-stage catalytic mechanism with drug design implications for rhomboid proteolysis

Intramembrane proteases signal by releasing proteins from the membrane, but despite their importance their enzymatic mechanisms remain obscure. We probed rhomboid proteases with reversible, mechanism-based inhibitors that allow precise kinetic analysis, and faithfully mimic the transition-state stru...

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Detalhes bibliográficos
Publicado no:Mol Cell
Main Authors: Cho, Sangwoo, Dickey, Seth W., Urban, Siniša
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4744120/
https://ncbi.nlm.nih.gov/pubmed/26805573
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2015.12.022
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