Quantitative Profiling of Post-translational Modifications by Immunoaffinity Enrichment and LC-MS/MS in Cancer Serum without Immunodepletion

A robust method was developed and optimized for enrichment and quantitative analysis of posttranslational modifications (PTMs) in serum/plasma samples by combining immunoaffinity purification and LC-MS/MS without depletion of abundant proteins. The method was used to survey serum samples of patients...

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Publicado en:Mol Cell Proteomics
Autores principales: Gu, Hongbo, Ren, Jian Min, Jia, Xiaoying, Levy, Tyler, Rikova, Klarisa, Yang, Vicky, Lee, Kimberly A., Stokes, Matthew P., Silva, Jeffrey C.
Formato: Artigo
Lenguaje:Inglês
Publicado: The American Society for Biochemistry and Molecular Biology 2016
Materias:
Technological Innovation and Resources
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4739682/
https://ncbi.nlm.nih.gov/pubmed/26635363
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.O115.052266
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