Auxin/AID versus conventional knockouts: distinguishing the roles of CENP-T/W in mitotic kinetochore assembly and stability

Most studies using knockout technologies to examine protein function have relied either on shutting off transcription (conventional conditional knockouts with tetracycline-regulated gene expression or gene disruption) or destroying the mature mRNA (RNAi technology). In both cases, the target protein...

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Vydáno v:Open Biol
Hlavní autoři: Wood, Laura, Booth, Daniel G., Vargiu, Giulia, Ohta, Shinya, deLima Alves, Flavia, Samejima, Kumiko, Fukagawa, Tatsuo, Rappsilber, Juri, Earnshaw, William C.
Médium: Artigo
Jazyk:Inglês
Vydáno: The Royal Society 2016
Témata:
Research
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4736828/
https://ncbi.nlm.nih.gov/pubmed/26791246
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1098/rsob.150230
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