Pancreatic cell tracing, lineage tagging and targeted genetic manipulations in multiple cell types using pancreatic ductal infusion of adeno-associated viral vectors and/or cell-tagging dyes

Genetic manipulations, with or without lineage tracing for specific pancreatic cell types, are very powerful tools for studying diabetes, pancreatitis and pancreatic cancer. Nevertheless, the use of Cre/loxP systems to conditionally activate or inactivate the expression of genes in a cell type– and/...

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Dettagli Bibliografici
Pubblicato in:Nat Protoc
Autori principali: Xiao, Xiangwei, Guo, Ping, Prasadan, Krishna, Shiota, Chiyo, Peirish, Lauren, Fischbach, Shane, Song, Zewen, Gaffar, Iljana, Wiersch, John, El-Gohary, Yousef, Husain, Sohail Z, Gittes, George K
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2014
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4734891/
https://ncbi.nlm.nih.gov/pubmed/25356582
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.183
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