Optimization of allele-specific PCR using patient specific HIV consensus sequences for primer design

Allele-specific PCR based on subtype consensus sequences is a powerful technique for detecting low frequency drug resistant mutants in HIV-1 infected patients. However, this approach can be limited by genetic variation in the region complementary to the primers, leading to variability in allele dete...

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Detalhes bibliográficos
Publicado no:J Virol Methods
Main Authors: Boltz, Valerie F., Maldarelli, Frank, Martinson, Neil, Morris, Lynn, McIntyre, James A., Gray, Glenda, Hopley, Mark J., Kimura, Toshio, Mayers, Douglas L., Robinson, Patrick, Mellors, John W., Coffin, John M., Palmer, Sarah E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4734888/
https://ncbi.nlm.nih.gov/pubmed/19948190
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2009.11.025
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