Optimization of allele-specific PCR using patient specific HIV consensus sequences for primer design

Allele-specific PCR based on subtype consensus sequences is a powerful technique for detecting low frequency drug resistant mutants in HIV-1 infected patients. However, this approach can be limited by genetic variation in the region complementary to the primers, leading to variability in allele dete...

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Bibliografische gegevens
Gepubliceerd in:J Virol Methods
Hoofdauteurs: Boltz, Valerie F., Maldarelli, Frank, Martinson, Neil, Morris, Lynn, McIntyre, James A., Gray, Glenda, Hopley, Mark J., Kimura, Toshio, Mayers, Douglas L., Robinson, Patrick, Mellors, John W., Coffin, John M., Palmer, Sarah E.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2009
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4734888/
https://ncbi.nlm.nih.gov/pubmed/19948190
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2009.11.025
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