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A Biophysical Model of CRISPR/Cas9 Activity for Rational Design of Genome Editing and Gene Regulation

The ability to precisely modify genomes and regulate specific genes will greatly accelerate several medical and engineering applications. The CRISPR/Cas9 (Type II) system binds and cuts DNA using guide RNAs, though the variables that control its on-target and off-target activity remain poorly charac...

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Publicat a:PLoS Comput Biol
Autors principals: Farasat, Iman, Salis, Howard M.
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2016
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4732943/
https://ncbi.nlm.nih.gov/pubmed/26824432
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pcbi.1004724
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