Real-time monitoring of NKCC2 endocytosis by total internal reflection fluorescence (TIRF) microscopy

The apical Na-K-2Cl cotransporter (NKCC2) mediates NaCl reabsorption by the thick ascending limb (TAL). The amount of NKCC2 at the apical membrane of TAL cells is determined by exocytic delivery, recycling, and endocytosis. Surface biotinylation allows measurement of NKCC2 endocytosis, but it has lo...

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Bibliographic Details
Published in:Am J Physiol Renal Physiol
Main Authors: Jaykumar, Ankita Bachhawat, Caceres, Paulo S., Sablaban, Ibrahim, Tannous, Bakhos A., Ortiz, Pablo A.
Format: Artigo
Language:Inglês
Published: American Physiological Society 2015
Subjects:
Innovative Methodology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4719042/
https://ncbi.nlm.nih.gov/pubmed/26538436
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1152/ajprenal.00104.2015
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