Real-time monitoring of NKCC2 endocytosis by total internal reflection fluorescence (TIRF) microscopy

The apical Na-K-2Cl cotransporter (NKCC2) mediates NaCl reabsorption by the thick ascending limb (TAL). The amount of NKCC2 at the apical membrane of TAL cells is determined by exocytic delivery, recycling, and endocytosis. Surface biotinylation allows measurement of NKCC2 endocytosis, but it has lo...

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Bibliografiske detaljer
Udgivet i:Am J Physiol Renal Physiol
Main Authors: Jaykumar, Ankita Bachhawat, Caceres, Paulo S., Sablaban, Ibrahim, Tannous, Bakhos A., Ortiz, Pablo A.
Format: Artigo
Sprog:Inglês
Udgivet: American Physiological Society 2015
Fag:
Innovative Methodology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4719042/
https://ncbi.nlm.nih.gov/pubmed/26538436
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1152/ajprenal.00104.2015
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