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Real-time monitoring of NKCC2 endocytosis by total internal reflection fluorescence (TIRF) microscopy
The apical Na-K-2Cl cotransporter (NKCC2) mediates NaCl reabsorption by the thick ascending limb (TAL). The amount of NKCC2 at the apical membrane of TAL cells is determined by exocytic delivery, recycling, and endocytosis. Surface biotinylation allows measurement of NKCC2 endocytosis, but it has lo...
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| Publicado no: | Am J Physiol Renal Physiol |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Physiological Society
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4719042/ https://ncbi.nlm.nih.gov/pubmed/26538436 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1152/ajprenal.00104.2015 |
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