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A Facile Stable-Isotope Dilution Method for Determination of Sphingosine Phosphate Lyase Activity

A new technique for quantifying sphingosine phosphate lyase activity in biological samples is described. In this procedure, 2-hydrazinoquinoline is used to convert (2E)-hexadecenal into the corresponding hydrazone derivative to improve ionization efficiency and selectivity of detection. Combined uti...

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Détails bibliographiques
Publié dans:Chem Phys Lipids
Auteurs principaux: Suh, Jung H., Eltanawy, Abeer, Rangan, Apoorva, Saba, Julie D.
Format: Artigo
Langue:Inglês
Publié: 2015
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4718852/
https://ncbi.nlm.nih.gov/pubmed/26408264
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chemphyslip.2015.09.006
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