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Downsizing a pullulanase to a small molecule with improved soluble expression and secretion efficiency in Escherichia coli
BACKGROUND: Significant challenges, including low expression and extracellular secretion of soluble protein, are encountered in expressing and purifying Bacillus acidopullulyticus pullulanase (BaPul) in Escherichia coli. METHODS: An N-terminal domain truncation was adopted to facilitate BaPul varian...
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| Publicado no: | Microb Cell Fact |
|---|---|
| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4712565/ https://ncbi.nlm.nih.gov/pubmed/26762529 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-015-0403-5 |
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