Serial femtosecond X-ray diffraction of enveloped virus microcrystals

Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with ∼700 Å diameter. Microcrystals delivered in viscous agarose...

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Detalhes bibliográficos
Publicado no:Struct Dyn
Main Authors: Lawrence, Robert M., Conrad, Chelsie E., Zatsepin, Nadia A., Grant, Thomas D., Liu, Haiguang, James, Daniel, Nelson, Garrett, Subramanian, Ganesh, Aquila, Andrew, Hunter, Mark S., Liang, Mengning, Boutet, Sébastien, Coe, Jesse, Spence, John C. H., Weierstall, Uwe, Liu, Wei, Fromme, Petra, Cherezov, Vadim, Hogue, Brenda G.
Formato: Artigo
Idioma:Inglês
Publicado em: American Crystallographic Association 2015
Assuntos:
SPECIAL TOPIC: BIOLOGY WITH X-RAY LASERS 2
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4711640/
https://ncbi.nlm.nih.gov/pubmed/26798819
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1063/1.4929410
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