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Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

Membrane proteins (MPs) are essential components of cellular membranes and primary drug targets. Rational drug design relies on precise structural information, typically obtained by crystallography; however MPs are difficult to crystallize. Recent progress in MP structural determination has benefite...

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Bibliographic Details
Published in:J Vis Exp
Main Authors: Ishchenko, Andrii, Cherezov, Vadim, Liu, Wei
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2016
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5092055/
https://ncbi.nlm.nih.gov/pubmed/27683972
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54463
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