Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography

Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously-renewed source of material for s...

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Hlavní autoři: Weierstall, Uwe, James, Daniel, Wang, Chong, White, Thomas A., Wang, Dingjie, Liu, Wei, Spence, John C.H., Doak, R. Bruce, Nelson, Garrett, Fromme, Petra, Fromme, Raimund, Grotjohann, Ingo, Kupitz, Christopher, Zatsepin, Nadia A., Liu, Haiguang, Basu, Shibom, Wacker, Daniel, Han, Gye Won, Katritch, Vsevolod, Boutet, Sébastien, Messerschmidt, Marc, Williams, Garth J., Koglin, Jason E., Seibert, M. Marvin, Klinker, Markus, Gati, Cornelius, Shoeman, Robert L., Barty, Anton, Chapman, Henry N., Kirian, Richard A., Beyerlein, Kenneth R., Stevens, Raymond C., Li, Dianfan, Shah, Syed T.A., Howe, Nicole, Caffrey, Martin, Cherezov, Vadim
Médium: Artigo
Jazyk:Inglês
Vydáno: 2014
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4061911/
https://ncbi.nlm.nih.gov/pubmed/24525480
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms4309
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