Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography

Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously-renewed source of material for s...

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Detalhes bibliográficos
Main Authors: Weierstall, Uwe, James, Daniel, Wang, Chong, White, Thomas A., Wang, Dingjie, Liu, Wei, Spence, John C.H., Doak, R. Bruce, Nelson, Garrett, Fromme, Petra, Fromme, Raimund, Grotjohann, Ingo, Kupitz, Christopher, Zatsepin, Nadia A., Liu, Haiguang, Basu, Shibom, Wacker, Daniel, Han, Gye Won, Katritch, Vsevolod, Boutet, Sébastien, Messerschmidt, Marc, Williams, Garth J., Koglin, Jason E., Seibert, M. Marvin, Klinker, Markus, Gati, Cornelius, Shoeman, Robert L., Barty, Anton, Chapman, Henry N., Kirian, Richard A., Beyerlein, Kenneth R., Stevens, Raymond C., Li, Dianfan, Shah, Syed T.A., Howe, Nicole, Caffrey, Martin, Cherezov, Vadim
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4061911/
https://ncbi.nlm.nih.gov/pubmed/24525480
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms4309
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