Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings

We present a protocol for determining the relative orientation and dynamics of A-form helices in (13)C/(15)N isotopically enriched RNA samples using NMR residual dipolar couplings (RDCs). Non-terminal Watson–Crick base pairs in helical stems are experimentally identified using NOE and trans-hydrogen...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Nat Protoc
Egile Nagusiak: Bailor, Maximillian H, Musselman, Catherine, Hansen, Alexandar L, Gulati, Kush, Patel, Dinshaw J, Al-Hashimi, Hashim M
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 2007
Gaiak:
Article
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC4707013/
https://ncbi.nlm.nih.gov/pubmed/17571061
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2007.221
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