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Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings
We present a protocol for determining the relative orientation and dynamics of A-form helices in (13)C/(15)N isotopically enriched RNA samples using NMR residual dipolar couplings (RDCs). Non-terminal Watson–Crick base pairs in helical stems are experimentally identified using NOE and trans-hydrogen...
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| izdano v: | Nat Protoc |
|---|---|
| Main Authors: | , , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2007
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4707013/ https://ncbi.nlm.nih.gov/pubmed/17571061 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2007.221 |
| Oznake: |
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