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Comparing the Affinity of GTPase-binding Proteins using Competition Assays
In this protocol we demonstrate a method for comparing the competition between GTPase-binding proteins. Such an approach is important for determining the binding capabilities of GTPases for two reasons: The fact that all interactions involve the same face of the GTPases means that binding events mus...
Gardado en:
| Publicado en: | J Vis Exp |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
MyJove Corporation
2015
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4705812/ https://ncbi.nlm.nih.gov/pubmed/26484896 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53254 |
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