Expression and phosphorylation state analysis of intracellular protein kinases using Multi-PK antibody and Phos-tag SDS-PAGE

Protein kinase expression and activity play important roles in diverse cellular functions through regulation of phosphorylation signaling. The most commonly used tools for detecting the protein kinase are protein kinase-specific antibodies, and phosphorylation site-specific antibodies were used for...

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Detalles Bibliográficos
Publicado en:MethodsX
Autores principales: Sugiyama, Yasunori, Katayama, Syouichi, Kameshita, Isamu, Morisawa, Keiko, Higuchi, Takuma, Todaka, Hiroshi, Kinoshita, Eiji, Kinoshita-Kikuta, Emiko, Koike, Tohru, Taniguchi, Taketoshi, Sakamoto, Shuji
Formato: Artigo
Lenguaje:Inglês
Publicado: Elsevier 2015
Materias:
Biochemistry, Genetics and Molecular Biology
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4703585/
https://ncbi.nlm.nih.gov/pubmed/26844212
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2015.11.007
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