Expression and phosphorylation state analysis of intracellular protein kinases using Multi-PK antibody and Phos-tag SDS-PAGE

Protein kinase expression and activity play important roles in diverse cellular functions through regulation of phosphorylation signaling. The most commonly used tools for detecting the protein kinase are protein kinase-specific antibodies, and phosphorylation site-specific antibodies were used for...

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Detalhes bibliográficos
Publicado no:MethodsX
Main Authors: Sugiyama, Yasunori, Katayama, Syouichi, Kameshita, Isamu, Morisawa, Keiko, Higuchi, Takuma, Todaka, Hiroshi, Kinoshita, Eiji, Kinoshita-Kikuta, Emiko, Koike, Tohru, Taniguchi, Taketoshi, Sakamoto, Shuji
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2015
Assuntos:
Biochemistry, Genetics and Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4703585/
https://ncbi.nlm.nih.gov/pubmed/26844212
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2015.11.007
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